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Part:BBa_K801005:Design

Designed by: Simon Heinze   Group: iGEM12_TU_Munich   (2012-10-02)


RFC25 MCS for optional addition of C-terminal Strep-Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 7
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 13
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23


Design Notes

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Source

This part has been rationally designed by Team TU Munich 2012. It has been physically prepared by oligo-hybridization.

References